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Erratum to: Co-culture of Retinal and Endothelial Cells Results in the Modulation of Genes Critical to Retinal Neovascularization
Vascular Cell volume 4, Article number: 6 (2012)
Correction
Following publication of our article [1] it was noted that Figures five E and five G were the same as Figures six A and six B. Figure 1 in this correction article is the correct version of Figure six that should have been included in the original article [1]. We apologize for any inconvenience caused by this error.
RT-PCR and Western blot analysis of NFκB. Total RNA and total protein were extracted from HRPC and HUVEC cultured alone or co-cultured under normoxia and hypoxia conditioned for 24 h. The expression of NFκB was measured by (A) electrophoresis of RT-PCR, (C) Western blot analysis in the HRPC and HUVEC. Figures (B, D) the band intensities corresponding to the NFκB were quantified by image analysis using a Bio-Rad scanning densitometer and Quantity One analysis software. Data were shown as ratio of NFκB densities to that of 18S RNA for RT-PCR and β-actin antibody was used to normalize Western blot for differences in loading and the transfer efficiencies. All data were expressed as mean +/- SE and results are representatives of three independent experiments.
References
Kumar R, Harris-Hooker S, Kumar R, Sanford G: Co-culture of Retinal and Endothelial Cells Results in the Modulation of Genes Critical to Retinal Neovascularization. Vascular Cell. 2011, 3: 27-10.1186/2045-824X-3-27.
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The online version of the original article can be found at 10.1186/2045-824X-3-27
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Kumar, R., Harris-Hooker, S., Kumar, R. et al. Erratum to: Co-culture of Retinal and Endothelial Cells Results in the Modulation of Genes Critical to Retinal Neovascularization. Vasc Cell 4, 6 (2012). https://doi.org/10.1186/2045-824X-4-6
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DOI: https://doi.org/10.1186/2045-824X-4-6