- Open Access
Inhibition of cyclo-oxygenase 2 reduces tumor metastasis and inflammatory signaling during blockade of vascular endothelial growth factor
- Jason C Fisher†1Email author,
- Jeffrey W Gander†2,
- Mary Jo Haley2,
- Sonia L Hernandez4,
- Jianzhong Huang2,
- Yan-Jung Chang2,
- Tessa B Johung2,
- Paolo Guarnieri5,
- Kathleen O'Toole3,
- Darrell J Yamashiro†2, 3, 4 and
- Jessica J Kandel†2
© Fisher et al; licensee BioMed Central Ltd. 2011
- Received: 13 April 2011
- Accepted: 6 October 2011
- Published: 6 October 2011
Vascular endothelial growth factor (VEGF) blockade is an effective therapy for human cancer, yet virtually all neoplasms resume primary tumor growth or metastasize during therapy. Mechanisms of progression have been proposed to include genes that control vascular remodeling and are elicited by hypoperfusion, such as the inducible enzyme cyclooxygenase-2 (COX-2). We have previously shown that COX-2 inhibition by the celecoxib analog SC236 attenuates perivascular stromal cell recruitment and tumor growth. We therefore examined the effect of combined SC236 and VEGF blockade, using the metastasizing orthotopic SKNEP1 model of pediatric cancer. Combined treatment perturbed tumor vessel remodeling and macrophage recruitment, but did not further limit primary tumor growth as compared to VEGF blockade alone. However, combining SC236 and VEGF inhibition significantly reduced the incidence of lung metastasis, suggesting a distinct effect on prometastatic mechanisms. We found that SC236 limited tumor cell viability and migration in vitro, with effects enhanced by hypoxia, but did not change tumor proliferation or matrix metalloproteinase expression in vivo. Gene set expression analysis (GSEA) indicated that the addition of SC236 to VEGF inhibition significantly reduced expression of gene sets linked to macrophage mobilization. Perivascular recruitment of macrophages induced by VEGF blockade was disrupted in tumors treated with combined VEGF- and COX-2-inhibition. Collectively, these findings suggest that during VEGF blockade COX-2 may restrict metastasis by limiting both prometastatic behaviors in individual tumor cells and mobilization of macrophages to the tumor vasculature.
Agents that inhibit vascular endothelial growth factor (VEGF) signaling are increasingly incorporated into treatment regimens for metastatic human cancer, yet the overall benefit of this treatment strategy has been relatively modest [1, 2]. Both clinical and experimental studies indicate that many or most malignancies will ultimately progress if VEGF blockade is sustained, and that progression may involve both progressive primary tumor growth and enhanced metastasis. The mechanisms for acquired resistance to this treatment approach are thus of great interest, but are still emerging. We previously found that VEGF inhibition significantly reduced primary tumor growth and the incidence of spontaneous lung metastasis in the orthotopic renal SKNEP1 tumor model over a six week treatment period, and regressed established metastases in late-stage tumors [3, 4]. Recent findings, however, indicate that disruption of VEGF signaling and consequent tumor hypoxia may ultimately promote invasion and metastasis in several tumor models [5, 6], overcoming the initial anti-metastatic effects of limiting angiogenesis. Prior studies suggest that hypoxia-regulated and proinflammatory genes expressed by tumor cells and stroma, such as COX-2, can promote the establishment of metastatic deposits in the lung. For example, Massague and coworkers previously found that COX-2 and other genes involved in vascular remodeling, identified as components in a "lung metastasis gene signature", functioned collectively to promote metastasis in a breast cancer model [7, 8]. More broadly, much recent data supports a role for systemic inflammation in the promotion of metastasis in general , including dissemination to the lung . For example, mice genetically prone to autoimmune arthritis are significantly more prone to develop lung metastasis than nonarthritic controls . Recruitment of COX-2-expressing macrophages can create an inflammatory proangiogenic environment that strongly promotes tumor growth .
Prior work demonstrates that the celecoxib analog SC236 can reduce spontaneous and experimental metastasis, although it is not clear whether this is due to effects on individual tumor cells, on the primary tumor (e.g. angiogenesis), or on the host environment . Thus, it is not known whether the addition of SC236 would limit spontaneous lung metastasis in hypoperfused tumors as occurs during VEGF blockade, when primary tumor angiogenesis is restricted but other prometastatic mechanisms may be active.
In previous studies in the SKNEP1 model, we found that treatment with SC236 perturbed tumor angiogenesis and reduced tumor weights . SC236 treatment resulted in the formation of erratic, segmentally dilated tumor vessels, marked by a decrease in early pericytes and a marked reduction in differentiated vascular mural cells (VMC). These alterations in vessel structure observed in SC236-treated xenografts differed strikingly from those previously found in this same model during administration of blocking anti-VEGF antibody . The potential importance of remodeling is supported by previous work indicating that periendothelial mural cells serve to protect endothelium during VEGF withdrawal , and by experiments showing that targeting VMC (e.g. by blockade of platelet-derived growth factor B signaling) in combination with VEGF inhibition enhances anti-angiogenesis [17, 18]. It is not known whether SC236 attenuates acquisition or function of perivascular stromal cells during VEGF inhibition, thus potentially reducing vessel stability.
In these studies, we used the SKNEP1 model to ask whether COX-2 inhibition might increase the efficacy of VEGF blockade, either by attenuating vessel remodeling, reducing primary tumor blood flow and tumor growth, or by improving control of metastasis. We asked whether restriction of metastasis could be mediated by direct effects of SC236 on SKNEP1 tumor cells, and whether these were altered in hypoxia, by studying tumor proliferation in vivo during SC236 and anti-VEGF treatment, and metabolic and invasive properties of tumor cells in vitro. We asked whether tumor expression of a subset of a lung metastasis signature containing COX-2 , and more broadly if expression of genes involved in macrophage recruitment, changed during SC236 treatment. Because these mechanisms may collectively contribute to tumor progression in conditions of reduced perfusion, understanding the effects of COX-2 inhibitors during VEGF blockade may hold promise for improving the usefulness of this treatment strategy.
The cell line SKNEP-1 (obtained from the American Type Culture Collection, Manassas, VA) was maintained in culture in 75-cm2 flasks with McCoy's 5A medium (Mediatech, Fisher Scientific, Springfield, NJ). Medium was supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Life Technologies, Grand Island, NY). Cells were grown at 37°C in 5% CO2 until confluent, harvested, counted with trypan blue staining, washed, and resuspended in sterile PBS (Life Technologies) at a concentration of 107 per mL.
The Institutional Animal Care and Use Committee of Columbia University approved all experiments. Four- to 6-week-old female NCR nude mice (National Cancer Institute at Frederick, Frederick, MD) were housed in a barrier facility and acclimated to 12-hour light/dark cycles for at least 24 hours before experimental use.
Mice (n = 62) were anesthetized with intraperitoneal ketamine (50 mg/kg) and xylazine (5 mg/kg). The left flank was prepared in a sterile fashion, and an incision was made, exposing the left kidney. An inoculum of 106 SKNEP1 tumor cells in 0.1 ml of PBS was injected into the renal parenchyma using a 25-gauge needle. The flank musculature was closed with a single 4-0 Polysorb suture (U.S. Surgical, Norwalk, CT), and the skin incision was closed with staples.
Administration of SC236 and anti-VEGF antibody
One week after tumor implantation, animals were divided into four cohorts. Treated mice received either (1) SC236 (N = 17; Pfizer, Groton, CT) added to drinking water at a concentration of 30 μg/mL and changed thrice per week as previously described ; (2) the humanized monoclonal anti-VEGF antibody bevacizumab (N = 17; BV; Genentech, South San Francisco, CA) injected intraperitoneally at 10 mg/kg biweekly beginning at week 3; or (3) combined agents (N = 15). Control mice (N = 13) received standard drinking water and were injected with carrier vehicle on the same schedule. This concentration of SC236 is equivalent to ~6 mg/kg/d (anticipated SC236 plasma level 5 μg/ml). Plasma levels of SC-236 were previously confirmed in our model by high-performance liquid chromatography (HPLC) .
Harvesting of specimens and determination of metastases
At sacrifice, tumors and contralateral kidneys were removed, weighed, and then preserved in 4% paraformaldehyde for immunohistochemistry. Portions of tumor were flash frozen in liquid nitrogen and stored at -80°C. Both lungs were fixed in 10% formalin for histology. Slides of paraffin-embedded lung tissue were stained by routine H&E methods and examined by a surgical pathologist to determine the presence or absence of metastases, using three levels from each lung.
Endothelial cell immunofluorescent staining was done using a rat anti-mouse anti-platelet-endothelial cell adhesion molecule-1 (PECAM-1) monoclonal antibody (MAP0032, Angioproteomie, Boston, MA). Vascular mural cells (VMC) were visualized using a rabbit anti-human α-smooth muscle actin (αSMA) antibody (RB-9010, Lab Vision/Neomarkers, Fremont, CA). Macrophages were visualized using an anti-murine F4/80 antibody (Abcam, AB6640) and an anti-arginase antibody (Santa Cruz Biotech, SC-20150). All microscopy was done using a Nikon Eclipse E600 apparatus.
Quantification of proliferation
In vivo tumor proliferation (N = 3 for all groups) was examined by immunofluorescent staining for anti-phospho-histone H3 (Upstate, Inc., Lake Placid, NY), and quantified by computer-assisted image analysis (23-30 fields per group examined).
MMP-2 and -9 zymography
Tumor extracts (N = 3 for each group) were normalized for protein content, mixed with nonreducing sample buffer, and electrophoresed in 10% polyacrylamide gels copolymerized with 1 mg/mL of gelatin. After electrophoresis, gels were washed twice for 15 min in 2.5% Triton X-100, rinsed with water, and incubated overnight in activation buffer (50 mM/L Tris-HCl, pH 7.5, 5 mM/L CaCl2) at 37°C. Digested gelatin bands were visualized by staining with 0.1% Coomassie blue R-250 in 40% methanol/20% acetic acid. Pooled MMPs were used as a positive control (US Biological). Bands were optically scanned for quantification.
2 × 105 SKNEP cells/well were incubated on a 24-well plate (BD Labware, Franklin Lakes, NJ) for 24 hours. Media was then replaced with control media or media with 25 or 50 uM SC-236 (Pfizer, New York, NY) and incubated for 24 or 96 hours at 37°C in either normoxia (21% oxygen) or hypoxia (2.3% oxygen). After the incubation period, media was aspirated and replaced with serum-free media and MTT labeling reagent (Roche, Indianapolis, IN). After 24 hours, 600 uL isopropanol was added to each well and the resultant solution read in a Life Science UV spectrophotometer (Beckman, Fullerton, CA) at wavelengths of 550 and 690 nm. All samples were plated in triplicate and experiments were repeated 3 times. Assays were repeated with added bevacizumab (100 ng/ml).
SKNEP cells were serum-starved for 24 hours. 5 uM Cell Tracker Green (Invitrogen) was added to the cells and incubated for 45 min. Media was then aspirated and cells incubated in serum-free McCoy's solution for an additional 45 min. 8 × 105 cells/well were plated in 24-well, 8 um pore, Matrigel-coated Tumor Invasion System plates (BD Biosciences) with serum-free media containing 0 (control), 25, or 50 uM SC-236. The same media with 15% FBS was added to the lower well, and the plate incubated for 24 hours in either normoxia or hypoxia (as above). Invading cells were quantified using a Cytofluor series 4000 Fluorescence multi-well plate reader (Applied Biosystems, Foster City, CA) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. All samples were plated in triplicate and experiments were repeated 3 times.
105 tumor cells per well were plated on a 96-well Microtest Tissue Culture Plate (BD Labware). Either media or media with 25 or 50 uM SC-236 was added, and allowed to incubate in either normoxia or hypoxia for 20 hours. Apoptosis was analyzed using the Cell Death Detection Elisa kit (Roche, Indianapolis, IN). Briefly, media was aspirated, and cells lysed. After a 30 minute incubation period, the solution was centrifuged at 1250 rpm for 10 minutes and the supernatant added to a streptavidin-coated plate. A mixture of anti-histone-biotin and anti-DNA-peroxidase solution was added to each well and mixed for 2 hours. Substrate was then added, and color intensity read using a VersaMax tunable microplate reader (Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. Camptothecin was used as a positive control for apoptosis.
Real-time PCR for lung metastasis signature genes
Total RNA was isolated from xenograft tumors (N = 4 each), using an Ambion ToTally RNA Extraction kit (Ambion, Austin, TX). Real-time PCR was performed using Taqman probes and an ABI 7300 (Applied Biosystems). Normalization of genes of interest was done utilizing the geNorm method described by Vandesompele et al . Six human housekeeping genes (ACTB, HMBS, UBC, GAPD, HPRT, PPIA) were used as reference controls.
Microarrays and gene set expression analysis
HG-U133A GeneChips (Affymetrix, Santa Clara, CA) were used to investigate gene expression in xenografts. cRNA probes were synthesized as recommended by Affymetrix. Briefly, total RNA was isolated in two steps using ToTALLY RNA Total RNA isolation kit (Ambion, Austin, TX) followed by RNeasy (Qiagen, Valencia, CA) purification. Double-stranded cDNA was generated from 5 μg of total RNA using a polydT oligonucleotide that contained a T7 RNA polymerase initiation site and the Superscript Choice System kit (Invitrogen, Carlsbad, CA). Biotinylated cRNA was generated by in vitro transcription using the Bio Array High Yield RNA Transcript Labeling System (Enzo, Farmingdale, NY). cRNA was purified using RNeasy and fragmented according to the Affymetrix protocol, and 15 μg of biotinylated cRNA hybridized to HGU133A microarrays (Affymetrix). Raw CEL files were processed using Bioconductor packages in an R environment http://www.bioconductor.org) . Briefly, quality controls were performed by inspecting Affymetrix® metrics using the simpleaffy package. Probe level signals were then background-corrected, normalized, and summarized using the GC-RMA function. Differential gene expression was computed using an Empirical Bayesian model implemented in the Limma package (Additional File 1 Table S1). To determine whether the addition of SC236 broadly changed inflammation-related pathways in BV-treated tumors, we used Gene Set Expression Analysis and GenePattern tools (GSEA, Broad Institute, Cambridge, MA). The MSigDB gene set database (5,542 total sets) was queried for gene sets containing inflammation-related pathways. From this, we constructed a 67 gene set matrix. GSEA was used to assess expression of gene sets in this matrix in BV- and BV+SC236-treated samples (N = 3, 4 respectively). To compute gene enrichment, we permuted by gene (as recommended for small sample sizes) 5000 times. We then computed odds ratios for the genesets identified as being significant by GSEA, defined as ratio of odds for the hits before and after the leading edge. In particular, for each geneset we computed the following:
oddsRatios =[(Hits/Misses) BeforeTheLeadingEdge]/[(Hits/Misses) AfterTheLeadingEdge]
We used the odds ratios to additionally filter the results, as nominal p-value is not informative in this setting, and the NES has a bias towards bigger genesets.
Enzyme-linked immunosorbent assay (ELISA)
ELISA for tumor-derived (human) IL4 was performed using the Human IL-4 Quantikine HS ELISA Kit HS400 (R&D Systems). All assays were performed in duplicate, and then repeated twice. Results of repeated assays were expressed as summary means ± standard error (square root of interassay variance) .
Tumor weights were compared by Kruskal-Wallis analysis. The presence or absence of lung metastases was evaluated by Fisher's exact test. Quantitative results of multiply-repeated MTT, invasion, and ELISA assays were assessed using Comprehensive Meta Analysis software (Biostat, Inc., Englewood, NJ). Differences in gene expression by real-time PCR were compared by Student's T-test.
Quantification of changes in vasculature
Digital images from immunofluorescence and fluorescein-labeled lectin studies were captured from a Nikon E600 fluorescence microscope with a Spot RT slider digital camera (Diagnostic Instruments, Sterling Heights, MI) and stored as TIFF files. Vessel radius was determined by immunofluorescence for αSMA and analyzed using software Adobe Photoshop 7.0 (Adobe Systems, Mountain View, CA) and Image Processing Toolkit (IPTK 5 IPTK 5, Reindeer Graphics, Raleigh, NC). Briefly, background fluorescence is subtracted (AutoLevelDark), Gaussian filter applied to reduce electronic and background noise (Gaussian Blur, radius = 1.0 pixel), and grayscale levels linearly expanded (Auto Levels). A common threshold level is applied that preserves correct vascular morphology (Threshold). The image is inverted (Invert) and dilated to lessen gaps (Classic Morphology > Dilate), small particles representing background removed (Reject Features), and vessel holes filled in (Fill Holes). The average of the inscribed radius is determined as a measure of vessel radius. 60 images from SK-NEP1-GFP (3 different tumors) and 49 images from SK-NEP1-Ang1* (3 different tumors) were analyzed. To assess MVD and vessel length, quantitative assessment of tumor vessel architecture was performed by computer-assisted digital image analysis as previously described . The fraction of fluorescein-labeled lectin-positive pixels per total field was quantified. Specific changes in vessel architecture are evaluated by quantifying branch points (nodes), end points, and total vessel length. Images are analyzed after application of a common threshold, image inversion, morphological erosion, and skeletonization, again using a combination of Photoshop and Image Processing Toolkit.
The addition of SC236 to VEGF inhibition does not further restrict tumor size, but does significantly reduce the incidence of metastasis as compared to anti-VEGF treatment alone
The combination of SC236 with VEGF inhibition alters vascular assembly as compared to either treatment alone
PECAM- and αSMA-immunostaining also suggested that SC236-treated xenografts were marked by erratically dilated larger vessels. To quantify this morphologic change, we measured shifts in distribution of the inscribed radius of mural cell-covered vasculature (Figure 2D, using a previously described method ). SC236-treated tumor vasculature demonstrated a significant increase in the vessels with inscribed radius > 10 μm.
SC236 affects proliferation and invasive properties of SKNEP1 tumors in vivo and in vitro
SC236 does not increase apoptosis in SKNEP1 cells in normoxia or hypoxia
COX-2 signaling can interfere with apoptotic mechanisms in some cell types and in some settings. Therefore, we examined the effect of SC236 on SKNEP1 cell apoptosis in vitro. No increase in apoptosis was found at either concentration of SC236, and no effect of hypoxia was detected (not shown). Thus, increasing apoptosis may not contribute to restriction of metastasis in this system.
The addition of SC236 does not alter expression of lung metastasis signature genes during VEGF blockade
We detected expression of 7 genes (COL6A1, CSF2RA, CXCR4, KRT81, MATN2, SPARC, TNC) included in the lung metastasis gene signature described by Minn et al.  in tumor extracts from each group (N = 6, controls, SC236-treated, BV-treated; N = 5, BV+SC236 treated; Additional File 3 Figure S2). Comparison was performed by real-time PCR. No significant differences between BV and SC236+BV treated tumors were found, suggesting that SC236 did not reduce lung metastasis by suppressing expression of these genes.
The addition of SC236 alters expression of gene sets representing macrophage-related inflammatory pathways in BV-treated tumors
The addition of SC236 alters expression of gene sets associated with macrophage recruitment pathways in BV-treated tumors
POSITIVELY ENRICHED GENE SETS
NEGATIVELY ENRICHED GENE SETS
Macrophage recruitment is altered by BV and SC236 treatment
While inhibition of VEGF signaling has become an established cancer therapy, it has become evident that its overall impact is relatively modest, with virtually all patients developing progressive disease [5, 6]. One proposed mechanism for the limitations of this approach is the alteration in tumor gene expression induced by hypoperfusion. Because it is regulated by hypoxia, a consequence of treatments that restrict blood supply, COX-2 may participate in this compensatory response to VEGF inhibitors. In addition, COX-2 signaling is implicated in multiple processes that contribute to tumor progression, including proliferation, survival, angiogenesis, and invasion. In these studies, we asked whether inhibition of COX-2 by SC236 would enhance the effects of VEGF blockade in the SKNEP1 model, either by perturbing compensatory vascular remodeling and primary tumor perfusion, or by further reducing the incidence of lung metastasis.
SC236 treatment significantly restricted primary tumor weight as compared to controls, as did BV. However, the addition of SC236 to BV treatment did not result in any further reduction, leading us to ask how this combination affected tumor vasculature and consequent tumor viability. As in our prior studies, both agents impaired angiogenesis, with reduction in branching and pruning of smaller vessels . SC236 disrupted vascular assembly, with erratic dilatation of large vessels. Tumors treated with combined agents displayed elements of these two patterns, with preserved VMC coverage of surviving vessels but irregular segmental dilatation and discontinuity. Tumor proliferation was not further reduced in vivo from treatment with SC236 alone. Thus, in our model the ability of SC236 to perturb VMC recruitment did not sufficiently destabilize vasculature during VEGF inhibition to further limit tumor growth.
In the SKNEP1 model, inhibition of VEGF reduces the incidence of lung metastasis, and can cause initial regression of established metastases in later-stage tumors . However, SKNEP1 tumors  and metastases (our unpublished observation) recur if VEGF inhibition is prolonged. Thus, our system may serve as an example of a tumor type in which metastasis is initially sensitive to VEGF blockade, in contrast to others in which it may actually be increased [5, 6]. The addition of SC236 to BV treatment did significantly improve control of metastasis, by reducing incidence. This finding suggests that SC236 may have limited one or more tumor cell behaviors that specifically contribute to establishment of metastasis, such as the ability to transit tumor parenchyma, invade vasculature, or survive as implants in the lung, distinct from effects on primary tumor growth. Consistent with this concept, proliferation in primary tumors was not further restricted in BV+SC236- as compared to SC236-treated xenografts. However, we found that viability of SKNEP1 cells (as assessed by MTT) tended to be restricted by SC236 in vitro in hypoxia, particularly if exposure were prolonged or at a higher concentration. It is possible that proliferation is supported in a primary tumor in vivo by secreted products of adjacent nonhypoxic regions, since BV+SC236 did not completely devascularize xenografts, or by host cells that contribute to tumor stroma. Invasion of tumor cells into basement membrane in vitro was similarly restricted by 50 μM SC236, with possible enhancement of this effect by hypoxia. However, while MMP-9 activity was reduced in both SC236- and BV-treated tumors, there was no further reduction in activity in BV+SC236-treated xenografts, suggesting that decreased invasion may not be mediated by diminished MMP-9. No effect of SC236 on tumor cell apoptosis was detected. Taken together, these findings are consistent with an effect on prometastatic behaviors (viability, invasion of vascular basement membrane) in individual tumor cells.
Because alteration in COX-2 signaling may affect tumor cell expression of other genes that specifically promote lung metastasis, we asked whether SC236 treatment altered expression of a subset of a previously described lung metastasis gene signature that includes COX-2 [7, 8]. While expression of these genes was detected in our model system, the addition of SC236 to BV did not reduce expression as compared to BV-treated tumors. More broadly, mobilization of macrophages by an inflammatory tumor microenvironment can also contribute to metastasis [9–11]. Given the ability of SC236 to block COX-2 activity, we asked whether the addition of SC236 to BV treatment limited primary tumor expression of gene sets implicated in macrophage-related pathways. Using gene set expression analysis, we found a shift toward significant suppression of gene sets containing cytokines that mediate such responses. In particular, we detected reduced expression of IL4 mRNA and protein. IL4 plays an established role in activating tumor-associated macrophages, and can be regulated by COX-2 activity. Suppression of this cytokine by COX-2 inhibition in the context of VEGF blockade might therefore contribute to attenuating a prometastatic tumor microenvironment. BV treatment quantitatively increased perivascular macrophage recruitment. The addition of SC236 did not limit this increase, or modulate distribution of cells bearing M1 or M2 markers. Thus, the effect of SC236 on tumor-associated macrophages in the context of VEGF blockade does not appear to include quantitative changes in recruitment.
Thus, our data provides evidence that inhibition of COX-2 may limit metastasis in the setting of VEGF inhibition, distinct from its effect on primary tumor angiogenesis and growth. Restriction of metastasis may result from reduced viability or ability to transit basement membrane in individual tumor cells. In addition, diminished primary tumor angiogenesis may have limited tumor cell access to the circulation, limiting hematogenous dissemination. We detected increased perivascular recruitment of macrophages in BV-treated tumors, and shifts in gene expression related to macrophage function between BV- and BV+SC236-treated tumors. However, we did not detect histologic changes in the distribution of macrophages when SC236 treatment was added to BV. Further investigation of functional effects of COX-2 blockade on interaction of tumor cells and stroma may assist in better understanding of these alterations, and in optimizing the use of anti-VEGF treatments in cancer patients at risk for metastatic progression.
These studies were supported by the National Institutes of Health (1R01CA124644, to DY, 1R01 CA100451 to JK), the Pediatric Cancer Foundation (to DY and JK), and the Sorkin Fund (to JK). Lucy Eljuga provided technical assistance.
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