Methylnaltrexone Potentiates the Anti-Angiogenic Effects of mTOR Inhibitors
© Singleton et al; licensee BioMed Central Ltd. 2010
Received: 7 June 2009
Accepted: 19 February 2010
Published: 19 February 2010
Recent cancer therapies include drugs that target both tumor growth and angiogenesis including mammalian target of rapamycin (mTOR) inhibitors. Since mTOR inhibitor therapy is associated with significant side effects, we examined potential agents that can reduce the therapeutic dose.
Methylnaltrexone (MNTX), a peripheral mu opioid receptor (MOR) antagonist, in combination with the mTOR inhibitors temsirolimus and/or rapamycin, was evaluated for inhibition of VEGF-induced human pulmonary microvascular endothelial cell (EC) proliferation and migration as well as in vivo angiogenesis (mouse Matrigel plug assay).
MNTX inhibited VEGF-induced EC proliferation and migration with an IC50 of ~100 nM. Adding 10 nM MNTX to EC shifted the IC50 of temsirolimus inhibition of VEGF-induced proliferation and migration from ~10 nM to ~1 nM and from ~50 to ~10 nM respectively. We observed similar effects with rapamycin. On a mechanistic level, we observed that MNTX increased EC plasma membrane-associated tyrosine phosphate activity. Inhibition of tyrosine phosphatase activity (3,4-dephostatin) blocked the synergy between MNTX and temsirolimus and increased VEGF-induced tyrosine phosphorylation of Src with enhanced PI3 kinase and mTOR Complex 2-dependent phosphorylation of Akt and subsequent activation of mTOR Complex 1 (rapamycin and temsirolimus target), while silencing Src, Akt or mTOR complex 2 components blocked VEGF-induced angiogenic events.
Our data indicate that MNTX exerts a synergistic effect with rapamycin and temsirolimus on inhibition of VEGF-induced human EC proliferation and migration and in vivo angiogenesis. Therefore, addition of MNTX could potentially lower the dose of mTOR inhibitors which could improve therapeutic index.
Recent therapeutic interventions for the inhibition of cancer progression include drugs that target both tumor growth and angiogenesis. Mammalian target of rapamycin (mTOR) inhibitors, including sirolimus (rapamycin) and temsirolimus, are potential therapeutic agents for hepatocellular cancer and renal cell carcinoma due to their anti-proliferative and anti-angiogenic properties. However, these mTOR inhibitors are often associated with unwanted side effects including rash, asthenia, mucositis, nausea, edema, anemia, hyperglycemia, thrombocytopenia, hyperlipaenia and anorexia [1–5]. Therefore, agents that can reduce the therapeutic concentration of these drugs could have significant clinical utility. We recently demonstrated that mu opioid agonists stimulate VEGF-induced angiogenesis via receptor transactivation and that mu opioid antagonists can inhibit VEGF receptor signaling . During the course of these investigations, we also noted an effect of the peripheral opiate antagonist methylnaltrexone (MNTX) on endothelial cell migration and proliferation that occurred beyond the VEGF receptor, through a mechanism that involves inhibition of Src and Akt. We therefore hypothesized that methylnaltrexone could have synergistic effects with anti-angiogenic drugs (i.e. mTOR inhibitors).
In this study, we demonstrate that methylnaltrexone (MNTX) acts synergistically with the mTOR inhibitors, rapamycin and temsirolimus, on inhibition of VEGF-induced angiogenic events. Specifically, MNTX inhibited EC proliferation with an IC50 of ~100 nM. Adding 10 nM MNTX shifted the IC50 of temsirolimus on EC proliferation from ~10 nM to ~1 nM. Further, adding 10 nM MNTX shifted the IC50 of temsirolimus on inhibition of EC migration from ~50 nM to ~10 nM. The synergistic effects of MNTX and temsirolimus were also demonstrated in an in vivo model of angiogenesis (mouse Matrigel plug assay). There was a shift in the IC50 on inhibition of VEGF-induced EC proliferation and migration with MNTX and rapamycin. The synergistic mechanism involves MNTX activation of tyrosine phosphatase activity with consequent inhibition of VEGF-induced Src activation. MNTX-induced Src inactivation results in inhibition of PI3 kinase and mTOR signaling required for Akt activation (serine/threonine phosphorylation). These results suggest addition of MNTX could potentially lower the therapeutic doses of mTOR inhibitors including rapamycin and temsirolimus.
Cell Culture and Reagents
Human pulmonary microvascular EC (HPMVEC) were obtained from Cambrex (Walkersville, MD) and cultured as previously described [7, 8] in EBM-2 complete medium (Cambrex) at 37°C in a humidified atmosphere of 5% CO2, 95% air, with passages 6-10 used for experimentation. Unless otherwise specified, reagents were obtained from Sigma (St. Louis, MO). Vascular endothelial growth factor (VEGF) was purchased from R&D Systems (Minneapolis, MN). Methylnaltrexone bromide or methylnaltrexone (MNTX) was purchased from Mallinckrodt Specialty Chemicals (Phillipsburg, NJ). Temsirolimus was acquired through Wyeth Pharmaceuticals. Rapamycin was purchased from Sigma (St. Louis, MO). Reagents for SDS-PAGE electrophoresis were purchased from Bio-Rad (Richmond, CA) and Immobilon-P transfer membrane was purchased from Millipore (Millipore Corp., Bedford, MA). Rabbit anti-pSer473Akt, rabbit anti-pThr308Akt, rabbit anti-Akt, rabbit anti-pThr389 p70 S6K and anti-p70 S6K antibodies were purchased from Cell Signaling Technologies (Danvers, MA). Rabbit anti-mTOR, rabbit anti-Rictor and rabbit anti-FKBP12 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Mouse anti-pp60src antibody was purchased from Upstate Biotechnologies (Lake Placid, NY). LY294002 was purchased from EMD Biosciences (Gibbstown, NJ). Mouse anti-β-actin antibody, rabbit anti-phospho-tyrosine418 Src antibody and naltrexone, were purchased from Sigma (St. Louis, MO). Secondary horseradish peroxidase (HRP)-labeled antibodies were purchased from Amersham Biosciences (Piscataway, NJ).
Immunoprecipitation and Immunoblotting
Cellular materials from treated or untreated HPMVEC were incubated with IP buffer (50 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM MgCl2, 1% Nonidet P-40 (NP-40), 0.4 mM Na3VO4, 40 mM NaF, 50 μM okadaic acid, 0.2 mM phenylmethylsulfonyl fluoride, 1:250 dilution of Calbiochem protease inhibitor mixture 3). The samples were then immunoprecipitated with either anti-Raptor or anti-Rictor IgG followed by SDS-PAGE in 4-15% polyacrylamide gels, transfer onto Immobilon™ membranes, and developed with specific primary and secondary antibodies. Visualization of immunoreactive bands was achieved using enhanced chemiluminescence (Amersham Biosciences).
Transfection of siRNA against mTOR, Src, Rictor, FKBP12 and Akt
The siRNA for human mTOR, Src, Rictor, FKBP12 and Akt were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and were utilized according to the manufacturer's specifications. Briefly, human lung EC were transfected with siRNA using siPORTamine™ as the transfection reagent (Ambion, TX). Cells (~40% confluent) were serum-starved for 1 hour followed by incubated with 250 nM of target siRNA (or scramble siRNA or no siRNA) for 6 hours in serum-free media. The serum-containing media was then added (1% serum final concentration) for 42 h before biochemical experiments and/or functional assays were conducted.
Human Pulmonary Microvascular EC Migration Assay
Twenty-four transwell units with 8 μM pore size were used for monitoring in vitro cell migration. HPMVEC (~1 × 104 cells/well) were plated with various treatments (MNTX, temsirolimus, LY294002, 3,4-Dephostatin or siRNA) to the upper chamber and VEGF (100 nM) was added to the lower chamber. Cells were allowed to migrate for 18 hours. Cells from the upper and lower chamber were quantitated using the CellTiter96™ MTS assay (Promega, San Luis Obispo, CA) and read at 492 nm. % migration was defined as the # of cells in the lower chamber % the number of cells in both the upper and lower chamber. Each assay was set up in triplicate, repeated at least five times and analyzed statistically by Student's t test (with statistical significance set at P < 0.05).
Human Pulmonary Microvascular EC Proliferation Assay
For measuring cell growth, HPMVEC [5 × 103 cells/well pretreated with various agents (MNTX, temsirolimus, LY294002, 3,4-Dephostatin or siRNA) were incubated with 0.2 ml of serum-free media containing 100 nM VEGF for 24 h at 37°C in 5%CO2/95% air in 96-well culture plates. The in vitro cell proliferation assay was analyzed by measuring increases in cell number using the CellTiter96™ MTS assay (Promega, San Luis Obispo, CA) and read at 492 nm. Each assay was set up in triplicate, repeated at least five times and analyzed statistically by Student's t test (with statistical significance set at P < 0.05).
Tyrosine Phosphatase Assay
HLMVEC were treated with MNTX (100 nM), VEGF (100 nM) and/or morphine (100 nM) for 5 minutes. Lysates were then obtained and further purified to enrich plasma membrane-associated proteins by wheat germ agglutinin (WGA) affinity as previously described  and analyzed for tyrosine phosphatase activity using the fluorometric Rediplate™ 96 EnzChek Tyrosine Phosphatase Assay Kit (Invitrogen (Molecular Probes), Eugene, OR) as we have previously described . Briefly, cellular materials are incubated in reaction buffer at 30°C and then added to a 96 well plate coated with 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP). Tyrosine phosphatase activity cleaves DiFMUP into DiFMU with an excitation/emission maxima of 358/452 nm.
In Vivo Angiogenesis Assay
The Matrigel plug assay was used to assess in vivo angiogenesis . 10-week-old female C57BL/6 mice (Jackson Laboratory, Bar Harbor, ME) were injected subcutaneously on the ventral abdomen with 500 μl Matrigel (BD Biosciences, San Jose, CA) containing either MNTX (100 nM), temsirolimus (10 nM), or both drugs (100 nM MNTX and 10 nM temsirolimus). 20 ng VEGF was added to all Matrigel plugs. After 21 days, the plugs were removed and analyzed for hemoglobin content. The plugs were weighed and homogenized, and their hemoglobin content was quantified using the QuantiChrom™ hemoglobin assay kit (BioAssay Systems, Hayward, CA).
Analysis of methylnaltrexone (MNTX) synergy with mTOR inhibitors on inhibition of human endothelial cell (EC) proliferation and migration
The roles of mTOR Complex components, Akt and Src in MNTX and temsirolimus inhibition of VEGF-induced angiogenesis
We and others have previously published that VEGF induces Src and PI3 kinase activation in human EC [6, 16, 17]. We inhibited PI3 kinase activity with LY294002 or silenced (siRNA) Src or Rictor (required for mTOR Complex 2 formation), challenged EC with VEGF and examined Akt activation. Our results (Figure 5-B) indicate that Src is required for both serine and threonine phosphorylation of Akt, the PI3 kinase pathway is required for threonine phosphorylation of Akt and mTOR Complex 2 is required for serine phosphorylation of Akt.
The role of tyrosine phosphatase activity in MNTX and temsirolimus inhibition of VEGF-mediated angiogenesis
In vivo analysis of MNTX synergy with temsirolimus on inhibition of angiogenesis
We have focused our studies on methylnaltrexone (MNTX) because it is more likely to be used in advanced illness clinical settings than tertiary mu opioid receptor antagonists. Uncharged mu opioid antagonists, including naloxone and naltrexone, are fairly lipid soluble and cross the blood-brain barrier easily [27–29]. Despite numerous attempts at regulating doses, mu opioid antagonists have proven unsuitable for patients receiving opiates for pain management because of analgesia reversal and breakthrough pain . MNTX is a quaternary derivative of the tertiary mu opiate antagonist naltrexone . The addition of the methyl group to naltrexone at the amine in the ring forms the compound N-methylnaltrexone with greater polarity and lower lipid solubility. Because MNTX does not cross the blood-brain barrier, it could play a therapeutic role in reversing the peripheral effects of opiates in palliative care, especially for patients taking high doses of opiates for analgesia [32–37]. The plasma concentrations of morphine and MNTX in patients after parenteral or oral administration are consistent with the levels that regulated synergistic inhibition of VEGF-induced angiogenesis and inhibited Src in our in vitro model (IC50 = ~10 nM) .
We focused our studies on temsirolimus and rapamycin based on our previous published data that MNTX regulates VEGF-induced Akt activation  and the intricate relationship between Akt and mTOR pathways [5, 26]. Both rapamycin and temsirolimus, a soluble ester analog of rapamycin, exert their action by binding to the intracellular protein, FKBP12, and inhibiting mTOR Complex 1 formation [38–40]. However, mTOR can still complex with SIN1 and Rictor (mTOR Complex 2) [1, 15, 26]. The mTOR Complex 2 serine phosphorylates Akt and is involved in actin cytoskeletal regulation [2, 4, 14, 41, 42]. Akt can also be threonine phosphorylated by PI3 kinase activation of PDK1 [41–45]. Activated (serine/threonine phosphorylated) Akt promotes mTOR Complex 1 assembly through inactivation (phosphorylation) of TSC2 and PRAS40 [5, 26]. Activated mTOR Complex 1 phosphorylates several target proteins including S6K and 4EBP1 involved in cell proliferation, growth and survival [1, 2, 4, 5, 26].
The effects of MNTX on inhibition of mTOR described in this manuscript go beyond VEGF receptor activation and extend to downstream signaling pathways. We and others have previously reported that inhibition of Src protects from EC barrier disruption and angiogenesis [6, 8, 10]. Src regulates several potential angiogenic events including EC contraction and vascular permeability [43, 44]. We extended these finding by observing that Src regulates VEGF-induced, PI3 kinase and mTOR-dependent, serine/threonine phosphorylation of Akt important for EC proliferation and migration. Further, Src regulates the synergistic effects of MNTX with temsirolimus on inhibition of VEGF-induced angiogenic events. We have previously demonstrated that MNTX increases tyrosine phosphatase activity, including RPTPμ . This study extends these finding by demonstrating that the potent protein tyrosine phosphatase inhibitor, 3,4-Dephostatin, blocks MNTX inhibition of VEGF-induced Src and Akt phosphorylation. 3,4-Dephostatin is known to block the phosphatase activity of PTP-1B, SHPTP-1 and CD45 [20, 40, 46–48]. In addition, 3,4-Dephostatin increased insulin-induced tyrosine phosphorylation of PLCγ, c-Cbl and the regulatory subunit of PI3 kinase (a known Src substrate) . We are currently examining the role of these tyrosine phosphatases in MNTX inhibition of VEGF-induced Src activation and angiogenesis.
Temsirolimus was approved by the FDA in 2007 for the treatment of advanced renal cell carcinoma, a disease resistant to existing chemotherapies (IFN-α, IL-2) [3, 38–40]. There have been other attempts to potentiate the action of temsirolimus. In Phase 3 clinical trails, temsirolimus, IFN-α or temsirolimus + IFN-α treatment resulted in median survival rates of 10.9 months, 7.3 months and 8.4 months, respectively [3, 38–40]. IFN-α did not augment temsirolimus treatment alone [3, 38–40]. The results of these clinical trials indicate the need for an effective drug in temsirolimus combination therapy. Our observations that MNTX acts synergistically with mTOR inhibitors on inhibition of VEGF-induced angiogenic events merit clinical studies.
Our results indicate that MNTX exerts a synergistic effect with rapamycin and temsirolimus on inhibition of VEGF-induced human EC proliferation and migration and in vivo angiogenesis. These synergistic effects are achieved through inhibition of different components of a common VEGF-induced angiogenic signaling pathway. MNTX inhibits the mu opioid receptor and stimulates tyrosine phosphatase activity which inhibits VEGF-induced Src activation and Src-regulated PI3 kinase and mTOR Complex 2-mediated Akt activation. Temsirolimus and rapamycin inhibit the downstream target of activated Akt, mTOR Complex 1. Inhibition of these events promotes synergistic inhibition of VEGF-induced angiogenesis (Figure 9). Therefore, addition of MNTX could potentially lower the dose of mTOR inhibitors which could improve therapeutic index.
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