Figure 1

Determination of methylnaltrexone (MNTX) synergistic effects with temsirolimus on inhibition of VEGF-induced human endothelial cell (EC) proliferation and migration. Panel A: Graphical representation of human EC assayed for VEGF (100 nM)-induced proliferation (24 hours) in the presence or absence of 0.1, 1.0, 10, 100 or 500 nM MNTX, temsirolimus, or temsirolimus + 10 nM MNTX. Experiments were performed in triplicate. Error bars = standard deviation. Panel B: Isobologram analysis of the combination of MNTX and temsirolimus on inhibition of VEGF-induced proliferation. The dashed line indicates a zero interaction for the isobole. The shift to the left indicates a synergistic interaction. Panel C: Graphical representation of human EC assayed for VEGF (100 nM)-induced migration (24 hours) in the presence or absence of 0.1, 1.0, 10, 100 or 500 nM MNTX, temsirolimus, or temsirolimus + 10 nM MNTX. Experiments were performed in triplicate. Error bars = standard deviation. Panel D: Isobologram analysis of the combination of MNTX and temsirolimus on inhibition of VEGF-induced migration. The dashed line indicates a zero interaction for the isobole. The shift to the left indicates a synergistic interaction.