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Fig. 4 | Vascular Cell

Fig. 4

From: A novel endothelial-derived anti-inflammatory activity significantly inhibits spontaneous choroidal neovascularisation in a mouse model

Fig. 4

ECPCM significantly decreases binding of p65 to E-selectin and VCAM-1 promoters upon TNFα treatment. HUVEC were treated for 1 h with or without TNFα in collection medium or ECPCM. The extracted chromatin was immunoprecipitated using anti-p65 antibody or isotype-matched IgG as control. The immunoprecipitated DNA was then quantified using real-time PCR. The level of p65 binding to the specific promoters was expressed as fold-change over the IgG control. p-value: * <0.01, ** <0.001 comparing ECPCM + TNFα to collection medium + TNFα treatment. Data = mean ± SEM

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Vascular Cell

ISSN: 2045-824X

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