Table 1 Antibodies for analysis of the pregnant mouse uterus
From: Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus
Name | Host | Company | Catalog Number | Concentration |
|---|---|---|---|---|
Primary antibodies | Â | Â | Â | |
 CD11b | Rat | Abcam | ab8878 | 1:500 |
 CD31 | Rat | BD Biosciences | 553370 | 1:350 |
 Desmin | Rabbit | Abcam | ab15200 | 1:500 |
 Dll4 | Goat | R&D Systems | AF1389 | 1:100 |
 F4/80 | Rat | eBioscience | 14-4801 | 1:100 |
 Jagged1 extracellular domain | Goat | R&D Systems | AF599 | 1:100 |
 Hey2 | Rabbit | Abcam | ab25404 | 1:200 |
 LYVE1 | Rabbit | Abcam | ab14917 | 1:1000 |
 NG2 | Rabbit | Millipore | AB5320 | 1:750 |
 Notch1 | Goat | R&D Systems | AF1057 | 1:200 |
 Notch2 | Rabbit | Abcam | Ab8926 | 1:500 |
 Notch3 | Rabbit | Abcam | Ab60087 | 1:50 |
 Notch4 Rb2-2 | Rabbit | Dr. Kitajewski’s laboratory |  | 1:100 |
 PDGFR-β | Rabbit | Cell Signaling | 3169 | 1:500 |
 PDGFR-β | Rat | Abcam | Ab91066 | 1:100 |
 α-SMA | Mouse | Sigma | C6198 | 1:850 |
Secondary antibodies | ||||
 Anti-goat-IgG Alexa-Fluor 594 | Donkey | Invitrogen | A11058 | 1:800 |
 Anti-rabbit-IgG Alexa-Fluor 594 | Donkey | Invitrogen | A21207 | 1:800 |
 Anti-rabbit-IgG Alexa-Fluor 488 | Donkey | Invitrogen | A21206 | 1:800 |
 Anti-rat-IgG Alexa-Fluor 594 | Donkey | Invitrogen | A21209 | 1:800 |
 Anti-rat-IgG Alexa-Fluor 488 | Donkey | Invitrogen | A21208 | 1:800 |