Fig. 12

Expression of Notch effector protein, Hey2, in relation to endothelial cells and mural cells in the peri-implantation uterus. H&E and double staining IF of E3.5 (A – C) and E6.5 (D – F) uterine sections. Ovals indicate areas of the uteri magnified (B1, C1, E1 and F1). (A) H&E at E3.5 highlighting the luminal epithelium (arrows), inner circular and outer longitudinal myometrium [dashed bracket (myo)], endometrial stroma (st) and serosa (s). (B, C) Hey2⁺ cells are observed throughout the myometrium and serosa. (B) Hey2 and CD31. Hey2 is not expressed in CD31⁺ ECs (B1). (C) Hey2 and SMA. Hey2 and SMA staining overlaps in the myometrium and serosa (C1). (D) H&E at E6.5 highlighting the embryo (e), inter-implantation sites (i), and myometrium (myo). (E, F) Hey2⁺ cells are observed throughout the myometrium. (E) Hey2 and CD31. Hey2 is not expressed in CD31⁺ ECs of decidual capillaries (E1). (F) Hey2 and SMA. Hey2 and SMA staining overlaps in the myometrium (F1). DAPI identifies all nuclei in IF images. am, anti-mesometrial; m, mesometrial. Bar in A and D =100 μm. Bar in B, C, E, and F =500 μm. Bar in B1, C1, E1 and F1 = 100 μm.