Figure 4
From: Delta-like 4 mRNA is regulated by adjacent natural antisense transcripts
Manipulation of Dll4-AS changes the expression of Dll4 . (A) pcDNA6.2-GW/EmGFP-Dll4-AS miR vectors were transfected into MS1 cells. qPCR was performed 48Â h later. Synthetic siRNAs were transfected into MS1 cells. qPCR (B) and immunostaining (C) were performed 48Â h later. pTracer-Dll4-AS vectors were transfected into MS1 cells. Western blotting (D) was performed 48Â h later.