Figure 7

Quantitative qRT-PCR of neural stem cell marker and SMC differentiation marker mRNA levels. A and B. Quiesced A7r5 and A10 cells were cultured in DMEM media supplemented with 5% FBS and 0.5% FBS for 3 d before relative gene expression of SMC markers [SMA, MHC and CNN1] and neural stem cell MVSC markers [Sox10, Sox17 and S100β] were determined by qRT-PCR. GAPDH was used to normalize gene expression. Data are mean ± SEM and are representative of three independent wells, *p < 0.01 when compared to 0.5% FCS. C. Representative growth curves for A10, A7r5 and MVSCs grown for 12 d in their respective media. Cell number was quantified by counting DAPI stained nuclei.