Skip to main content
Figure 1 | Vascular Cell

Figure 1

From: Previously differentiated medial vascular smooth muscle cells contribute to neointima formation following vascular injury

Figure 1

Schematic representation of the mTmG reporter strain. The mTmG reporter mice (Jackson strain: B6.129 (Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J ) contain a single copy of the transgene integrated into the ROSA 26 locus. The transgene cassette is comprised of a chimeric CMV, β-actin promoter driving the expression of a floxed membrane localized Tomato tandem dimer. Following cre-mediated excision the mTomato transgene is removed such that the CAG promoter now drives expression of membrane localized EGFP. The reporter mice were crossed with transgenic mice that express a tamoxifen regulated cre recombinase directed to smooth muscle cells by either the Myh11 promoter or the Acta2 promoter. Double heterozygous transgenic mice were used for all experiments.

Back to article page

Vascular Cell

ISSN: 2045-824X

Contact us