Figure 7

CSF-1R^highcells preferentially accumulate higher levels of LysoTracker. (A) COSB and AS5 were stained for cell surface CSF-1R expression using an RPE-labeled CSF-1R antibody followed by incubation with increasing concentrations of LysoTracker DeepRed. LysoTracker fluorescence was assessed in the CSF-1R^high cell population (approximately 1-2% of all cells), and this fluorescence was compared to the CSF-1R^low cell population (dimmest 3-5% of all cells) in each cell line. The fluorescence intensity of cells without LysoTracker or with increasing concentrations of LysoTracker added to the CSF-1R^low and CSF-1R^high cell populations is shown. For the AS5 studies, the coefficient of variation (CV) values are provided as [LysoTracker concentration (CSF-1R^low, CSF-1R^high)] for comparison of the histograms: [0 nm (39.4, 88.9); 5 nm (61.2, 109); 25 nM (78.3, 97.6); 50 nM (65.2, 86.3] (B) The relative fluorescence levels for each population compared to the untreated controls were plotted against the increasing LysoTracker concentrations. The graphs represent the data presented from each cell line in panel A.