Figure 5

VEGFR-1 blockade reduces peri-implantation macrophage and vascular density at E7.5. (A, B) Double staining for F4/80 (green) and CD31 (red) in E7.5 uterine frontal sections from mice treated with VEGFR-1 blocking antibodies (MF-1 or AF471) or from control mice. Reduced expression of F4/80 and CD31 is observed in treatment groups. Scale bars = 50 μm. (C, D) F4/80⁺ or CD31⁺ signal was determined and normalized by total decidual area. F4/80⁺ macrophage and CD31⁺ EC density is significantly reduced in uteri of mice that received MF-1 compared to controls.