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Table 2 Real time PCR analysis of the expression of mRNA for the integrin subunits α v , β 3 and β 5 in HUVEC cultured for 5 h in basal conditions and with VEGF, EGF, IGF, and FGF, alone (control) or in the presence of 1 μM cyclo [DKP-RGD] 1

From: Effects of a novel cyclic RGD peptidomimetic on cell proliferation, migration and angiogenic activity in human endothelial cells

Subunit control + cyclo[DKP-RGD] 1
  2 -Δct ×10 2 2 -Δct ×10 2 Ratio vs Control P vs Control
A. Basal conditions
αv 6.65 ± 6.41 6.59 ± 5.24 1.32 ± 0.57 0.944
β3 0.89 ± 0.87 0.91 ± 0.76 1.08 ± 0.27 0.928
β5 1.36 ± 1.18 1.52 ± 1.34 1.08 ± 0.07 0.225
B. With VEGF, EGF, IGF, and FGF
αv 13.31 ± 12.80 15.09 ± 10.66 1.28 ± 0.42 0.494
β3 1.15 ± 1.07 1.41 ± 1.07 1.43 ± 0.35 0.288
β5 1.23 ± 1.25 1.31 ± 1.13 1.34 ± 0.46 0.461
  1. Data are means ± SD of 3 separate experiments.