Figure 5

DCC, Neogenin and Unc5B are required for Netrin-4-induced VSMC adhesion. VSMC were transfected with a control non-specific siRNA (control; A & B) or a specific siRNA targeting the DCC (C), Neogenin (D) or Unc5B (E) receptors. Contrast phase images indicate that transfected VSMC adhered to Vitronectin (A) and to Netrin-4 (B-E). Scale bar: 150 μm. (F) Quantification of cell adhesion to the three substrates by measurement of absorbance under the four experimental conditions. Silencing of DCC, Neogenin or Unc5B significantly decreased VSMC adhesion to Netrin-4, implicating these three receptors in the process.