Figure 1
From: Netrin-4 promotes mural cell adhesion and recruitment to endothelial cells
Netrin-4 promotes Mural cell migration. Time course of Vascular Smooth Muscle Cell (A) and Porcine Retinal Pericyte Cells (B) migration with and without the addition of Netrin-4 (R&D; 50 ng/ml). Cells were grown to confluence in complete medium and wounds were made. Cells were treated (N4) or not (Control) with the human recombinant protein Netrin-4 (50 ng/ml) and allowed to migrate for 20 hours. Data were collected and analyzed on IncuCyte. Compared to controls, treatment with Netrin-4 stimulates VSMC (A) and PRPC (B) migration. Statistical analysis of data at t = 10 h and t = 20 h showed that Netrin-4 significantly increased VSMC (A) and PRPC (B) migration.