Figure 4

Vascular perfusion is compromised in tumors developed in Notch4^−/−hosts whereas vessel density is unaffected. (a) CD31 immunostaining (green) and lectin-perfused (red) blood vessel patterns in orthotopic MMTV-PyMT tumors are similar in wild type (i) and Notch4^−/− (ii) hosts. (b) Vessel perfusion in MMTV-PyMT tumors is decreased in Notch4^−/− hosts compared to wild type hosts 9 days after orthotopic transplantation. (c and d) Vessel density, either measured as number of vessel segments (c) or as number of branch points (d) in MMTV-PyMT tumors is similar in wild type and Notch4^−/− hosts 9 days after orthotopic transplantation. (e) CD31 staining in tumors developed in wild type (i) and Notch4^−/− (ii) hosts 2 weeks after transplantation. (f) Vessel density in MMTV-PyMT tumors is similar in wild type and Notch4^−/− hosts 2 weeks after orthotopic transplantation. Bars represent average values and error bars represent s.e.m.