Figure 1
From: Notch and VEGF pathways play distinct but complementary roles in tumor angiogenesis
Combined Notch and VEGF increases tumor necrosis and apoptosis. (A) NGP-N1D tumors did not have different tumor weights than NGP-LacZ controls. BV treatment reduced tumor weights compared to controls and NGP-Notch1 decoy. Combining Notch1 decoy with BV reduced tumor weights compared to NGP-LacZ controls and NGP-N1D Error bars represent SD. ***P < 0.001. (B) NGP-N1D + BV tumors had increased necrosis as seen by H&E. Bar = 400 μm. (C) Quantification of necrosis showed NGP-N1D + BV with increased necrosis. Error bars represent SD. **P < 0.01, ***P < 0.001. (D) NGP-N1D and NGP-LacZ + BV tumors had increased numbers of TUNEL positive counts per field compared to NGP-LacZ controls. An additive increase was observed in NGP-N1D + BV tumors (p < 0.001). Error bars represent SD. *P < 0.05, **P < 0.01, ***P < 0.001. (E) Proliferation in tumors was assessed by phosphorylated-Histone H3 (H3-P) IHC. There was no significant difference in proliferation. Error bars represent SD. P = n.s.