Figure 5
From: RhoB controls endothelial cell morphogenesis in part via negative regulation of RhoA
Inhibition of ROCK partially restores capillary morphogenesis in RhoB-depleted HUVEC. Cells were transfected with siRNAs as indicated and seeded onto BME in EGM-2 growth media in the absence or presence of (A) 10 μM Y-27632 or (B) 1 μM H-1152. In both cases, control media conditions contained EGM-2 growth media with equivalent volumes of DMSO as a vehicle control. Images were taken 24 h after cell seeding. (C, D) Analysis of capillary morphogenesis from (A, B). Capillary morphogenesis was determined by averaging the total number of cord-like structures in 4 fields of view as assessed with ImageJ software. Bars represent the mean ± SE of duplicate wells from each of two independently performed experiments (* represents p < 0.05 and ** represents p < 0.0001 as compared to the control siRNA sample using unpaired t-tests).