Figure 5

Production of VE-cadherin in HUVECs (A) RT-PCR: Cells were maintained in culture in MCDB 131 medium for different time intervals. Total RNA was isolated and subjected to RT-PCR as described in the text. Intensity of bands were quantitated and normalized with the intensity of band for internal control GAPDH and expressed in intensity units/mm² (B). * Statistically significant when compared to their production on first day p < 0.05. C. Western blotting HUVECs were maintained in culture in MCDB 131 medium for different time intervals. Medium was removed, the cells were harvested and lysed in Laemmeli buffer and protein equivalent amount of the cell lysates were subjected to SDS PAGE (bottom) and western blot (top) analysis with anti VE-cadherin antibody as described in the text. Inset, Lane1-4 represents 1^st -4^th day. The intensity of the immunoblotted bands were measured and expressed in intensity units/mm²/mg protein. * Statistically significant when compared to the production on first day p < 0.05.