Figure 4

Effect of supplementation of curcumin in presence of serum on the production and activities of MMP-2 and MMP-9: (A) Equivalent aliquots of the medium appropriately diluted (1:10)from the culture of FCS control and curcumin +FCS treated cells on days 1 to 4 were normalized to the total cell protein were subjected to zymography as described in the text. (a) Lane1-4 curcumin + FCS treated cells 1^st -4^th day, Lane 5-9 control 1^st-4^th day. The activity of MMP-9(b) and MMP-2(c) expressed in arbitrary units of intensity/mm²/mg protein. Values given are the average of intensity measurement of 6 experiments ± SEM. (B) ELISA The culture medium collected from controls and curcumin treated cells in presence of serum at intervals of 24 hrs which were normalized to the total cell protein were quantitated for MMP-9(d) and MMP-2(e) protein expression by ELISA using specific antibody. Values given are the average of duplicate analysis of 5 experiments ± SEM. * Statistically significant when compared to their production on first day p < 0.05. # Statistically significant when compared to control p < 0.05.