Figure 5

Changes in miR-155, miR-100, and miR-let-7i expression affect in vitro endothelial morphogenesis. 24 hours after transfection with specific synthetic inhibitors (A) or mimics (B), ECs were subjected to in vitro EC morphogenesis assay. DIC images of unlabeled cells were acquired using a Nikon TE2000 microscope and SlideBook software (DIC micrographs). GAPDH-labeled low-magnification images of the tubular structures (red, inserts) were acquired using a Zeiss LSM confocal microscope. Bars 20 μm. Graphs: Data are expressed as the average tube length, or number of branch points per visual area ± S.E.M., n = 10 wells per inhibitor/10 images per well. Student's t-test: * P < 0.05; **P < 0.01; *** P < 0.001.