Figure 4

Endothelial differentiation of stem cells. (A) VEGFR2, (B) VE-cadherin and (C) CD31 expression in ESCs, relative to 60% confluent ECs (± SEM; n = 3). Dotted line indicates expression in 60% confluent EPCs. Directed differentiation with ECCM, significant differences between treatments indicated (*P < 0.05, **P < 0.01). (D): VEGFR2, CD133 and CD34 proteins in ECCM-treated ESCs. Scale bars = 10 μm.