Figure 5
From: A role for Egfl7 during endothelial organization in the embryoid body model system
Effect of Egfl7 knock-down on embryoid body proliferation and apoptosis. Cryosections of EBs differentiated for 7 or 14 days were subjected to indirect IF using antibodies against CD31 plus Ki67 (40× magnification used; upper panels, a-d), or CD31 plus Annexin V (20× magnification used; bottom panels, f-i), (arrow, CD31+ cord; arrowhead, CD31+ sheet; asterisks, Annexin V+ cells). IgG controls are shown at 40× and 20× magnification (e, j). Images were acquired using an Axioplan 2 imaging microscope (Carl Zeiss).