Figure 3

Reduced CLIC1 expression inhibits endothelial migration. A. CLIC1 knockdown endothelial lines exhibited inhibited directed migration in a scratch assay, noticeable as early as 3 h post-wounding. Photographs shown here have been analyzed using the TScratch software for quantifying open surface area. Lighter shaded areas indicate area occupied by endothelial cells while darker shaded areas indicate surface area not occupied by endothelial cells. Scale bar, 50 μm. B. Quantification using the TScratch software showed that there was significantly more open surface area in the CLIC1 knockdown group at and after 6 h post-wounding. Mean open surface area for each group is shown with standard deviation. (*p < 0.02; **p < 0.001).