Figure 1

Previous HHT3 interval. A) HHT3 linked Family S pedigree (black symbols represent HHT-affected individuals; white symbols represent unaffected individuals; squares represent males and circles represent females. Individuals are denoted by their generation number, such that the oldest male is I.1, oldest female I.2). B) Published HHT3 interval on chromosome 5 between microsatellite markers D5S2011 and D5S2490 [35], identifying the region shared by all affected individuals (open interval between D5S402 and D5S436); regions where the designated individuals had inherited their affected parent's non disease-gene bearing allele (black bars), and regions in which the transition from the non disease-gene bearing allele (black bars) to disease-gene bearing allele (open interval) had occurred, but the exact sites of the meiotic recombination event was not definable using the markers studied in [35] (grey bars; uninformative markers). It was possible that the interval could be reduced to a minimum of D5S402-D5S436, according to where the recombination breakpoints had occurred in individuals II.1, II.3 and II.9. cen: centromere; tel: telomere; Mb: mega bases.