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Fig. 7 | Vascular Cell

Fig. 7

From: Angiogenic and anti-inflammatory properties of micro-fragmented fat tissue and its derived mesenchymal stromal cells

Fig. 7

LG-CM and LG-MSCs possess anti-inflammatory properties. The capacity of LG-CM and LG-MSCs to affect inflammatory cell functions was tested on the U937 monocyte cell line. In (a) the effect of LG-CM and LG-MSCs-CM on U937 migration. Different dilutions of LG-CM and LG-MSCs-CM were placed in the lower compartment of a transwell insert (5um pore size). The chemokine MCP-1 at (10 ng/ml) was used as a positive stimuli for U937 migration. RPMI+ 0.2 % BSA was the control medium (CTRL) (* p < 0.05; ** p < 0.01 versus CTRL; °p < 0.05, versus MCP-1 treatment). In (b) adhesion of U937 to HUVECs monolayer in the presence of LG-CM and LG-MSCs-CM. TNFα at 20 ng/ml was used to activate HUVECs (* p < 0.05; ** p < 0.01 versus CTRL; °p < 0.05, °° p < 0.01 versus TNFα treatment). The photos below the figure show the morphological appearance of U937 attached to HUVECs primed with TNFα, LG-CM and LG-MSCs-CM after 30’ incubation (5x magnifications). In (c) and in (d) the release of RANTES and MCP-1 by U937 under the influence of LG-CM and LG-MSCs-CM stimulated or not with LPS (1ug/ml) respectively (*p < 0.05; **p < 0.01 versus CTRL; °p < 0.05, °° p < 0.01 versus LPS treatment)

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