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Fig. 5 | Vascular Cell

Fig. 5

From: A novel endothelial-derived anti-inflammatory activity significantly inhibits spontaneous choroidal neovascularisation in a mouse model

Fig. 5

ECPCM reduces CNV area and macrophage recruitment to CNV lesions. JR5558 mice received daily intraperitoneal injections of ECPCM or PBS from P22 (day 1) to P28 (day 7). a Representative early phase fluorescein angiography images obtained from JR5558 mice before (P21, day 0) and after (P29, day 8) 7 days of treatment with either PBS or ECPCM. b CNV area/retina was determined at baseline (day 0) and after the treatment (day 8) using fluorescein angiography. The graph shows the % increase or decrease in CNV area/retina at day 8 compared to day 0 (+5.9 % for PBS and −29.89 % for ECPCM treatment). p-value: * <0.05. Data = mean ± SEM. c After 7 days of treatment with ECPCM or PBS, eyecups were collected and stained with anti-F4/80 antibody for activated macrophages (green) and anti-PECAM-1 antibody for EC (red) of the CNV. Two representative images for each group of treatment are shown. d Quantification of macrophage recruitment at CNV lesions expressed as macrophage number around each lesion. p-value: * <0.05. Data = mean ± SEM

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