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Fig. 9 | Vascular Cell

Fig. 9

From: Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus

Fig. 9

Expression of Notch ligand, Dll4 in relation to endothelial cells and mural cells in the peri-implantation uterus. H&E and double staining IF of E3.5 (A – D) and E6.5 (E – H) uterine sections. Ovals indicate areas of the uteri magnified (B1 – D1, F1 – H1). (A) H&E at E3.5 highlighting the luminal epithelium (arrows), inner circular and outer longitudinal myometrium [dashed bracket (myo)], endometrial stroma (st) and serosa (s). (B – D) Dll4+ cells are scattered throughout the stroma, myometrium (box) and serosa. (B) Dll4 and CD31. Dll4 is expressed in CD31+ ECs in the endometrial stroma. (C) Dll4 and NG2. NG2+ mural cells are observed adjacent to the Dll4+ cells in the stroma. (D) Dll4 and PDGFR-β. PDGFR-β+ mural cells surround Dll4+ stromal cells. (E) H&E at E6.5 highlighting the embryo (e), inter-implantation sites (i), and myometrium (myo). (F – H) At E6.5, Dll4+ cells are observed throughout the decidua, with most Dll4 expression in the anti-mesometrial decidua. (F) Dll4 and CD31. Dll4 is expressed in CD31+ decidual ECs. (G) Dll4 and NG2. NG2+ mural cells are adjacent to Dll4+ decidual cells in the anti-mesometrium. (H) Dll4 and PDGFR-β. PDGFR-β+ mural cells are adjacent to Dll4+ decidual cells in the anti-mesometrium. DAPI identifies all nuclei in IF images. Bar in A and E =100 μm. Bar in B – D and F – H =500 μm. Bar in B1 – D1 and F1 – H1 = 50 μm.

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