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Fig. 8 | Vascular Cell

Fig. 8

From: Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus

Fig. 8

Location of Notch3 (N3) in relation to endothelial cells and mural cells in the peri-implantation uterus. H&E and double staining IF of E3.5 (A – D) and E6.5 (E – H) uterine sections. Ovals indicate areas of the uteri magnified (B1 – D1, F1 – H1). (A) H&E at E3.5 highlighting the luminal epithelium (arrows), inner circular and outer longitudinal myometrium [dashed bracket (myo)], endometrial stroma (st) and serosa (s). (B – D) Notch3+ cells are observed throughout the myometrium and serosa and in endometrial glands (g). (B) Notch3 and CD31. Notch3 is not expressed in CD31+ ECs. (C) Notch3 and PDGFR-β. Notch3 is expressed in PDGFR-β+ mural cells in the endometrial stroma (arrowheads in C1). (D) Notch3 and SMA. Notch3 and SMA staining overlaps in the myometrium and serosa. (E) H&E at E6.5 highlighting the embryo (e), inter-implantation sites (i), and myometrium (myo). (F – H) Notch3+ cells are observed throughout the myometrium. (F) Notch3 and CD31. Notch3 is not expressed in CD31+ ECs. (G) Notch3 and PDGFR-β. Notch3 is not associated with PDGFR-β in the decidua or myometrium. (H) Notch3 and SMA. Notch3 and SMA staining overlaps in the myometrium. DAPI identifies all nuclei in IF images. am, anti-mesometrial; m, mesometrial. Bar in A and E =100 μm. Bar in B – D and F – H =500 μm. Bar in B1 – D1 and F1 – H1 = 50 μm.

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