Fig. 11

Notch activity in endothelial cells and mural cells in the peri-implantation uterus. (A) H&E at E3.5 highlighting the luminal epithelium (arrows), inner circular and outer longitudinal myometrium [dashed bracket (myo)] and endometrial stroma (st). (B, C) IF of sections through the uterus of CBF:H2B-Venus Notch reporter mice enabled assessment of Notch activity at E3.5. Ovals indicate areas of the uteri magnified (B1, C1). (B) Notch activity was observed in CD31⁺ ECs (red) in the stroma (B1), myometrium, and glandular epithelium (arrowheads). (C) Notch activity was observed in PDGFR-β⁺ mural cells in the stroma (C1) and myometrium. (D) H&E at E6.5 highlighting the embryo (e), inter-implantation sites (i), and myometrium (myo). (E, F) IF of sections through implantation sites of female CBF:H2B-Venus Notch reporter mice at E6.5. Ovals indicate areas of the uteri magnified (E1, F1). (E) Notch activity was observed in CD31⁺ ECs of decidual capillaries (E1) and myometrial vessels. (F) Notch activity was observed in PDGFR-β⁺ mural cells in the anti-mesometrial decidua. DAPI identifies all nuclei in IF images. am, anti-mesometrial; m, mesometrial. Bar in A and D =100 μm. Bar in B, C, E, and F = 500 μm. Bar in B1, C1, E1, and F1 = 100 μm.