Figure 5

Solenopsin A and analogs increase superoxide levels. Investigation of solenopsin and analogs S11-S14 effect on superoxide levels in (a) A375 cells, and (b) SVR cells. Cells were treated for 24 h with 10 μM of (+)-solenopsin A ((+) Sol. A), (−)-solenopsin A ((−) Sol. A), and solenopsin analogs S11-S15. Cells were trypsined and incubated for 10 min in 10 μM dihydroethedium, followed by analysis with a FACScan flow cytometer. Data shown are an average of three experiments ± s.d.