Figure 3
From: Delta-like 4 mRNA is regulated by adjacent natural antisense transcripts
Dll4-AS responses to exogenous stimuli. (A) Dll4-AS expression coincides with Dll4 in the status of different cellular confluences. Dll4-AS expression level was measured in 0.8%, 4%, 20% and 100% confluent MS1 cells by quantitative PCRs. (B) DAPT treatment down-regulates the expression of both Dll4-AS and Dll4 in MS1 cells. (C) HUVECs deprived of serum for 16 h were treated with 50 ng/ml recombinant human VEGF or FGF for 24 h. DLL4 and DLL4-AS were quantitated by qPCR in these cells. (D) HCAECs deprived of serum for 16 h were treated with 50 ng/ml recombinant human VEGF or FGF for 24 h. DLL4 and DLL4-AS were quantitated by qPCR in these cells. (E) 6-day old C57/BL6 mice were treated with 1 mg/kg intraperitoneal LPS. Dll4 and Dll4-AS were quantitated by qPCR in neonatal mouse lungs 18 hr after systemic LPS. *denotes p < 0.05.