Purification and activity of
PADS. a. SDS-PAGE gel with silver staining demonstrating the purity of VEGFPADS and ELP control polypeptides. Lane 1, ELP; Lane 2, VEGFPADS; Lane 3, VEGF121. b. HUVEC cell proliferation was determined after 72h exposure to ELP, VEGF, or VEGFPADS at the indicated concentrations using the MTS cell proliferation assay. *Statistically significant increase versus untreated cells (p = 0.0003, two-way ANOVA with post-hoc Bonferroni multiple comparison).