Figure 4
From: The role of RNA interference in the developmental separation of blood and lymphatic vasculature
Mutant embryos present blood-filled lymphatics. A) Histological analysis of E13.5 WT and dicerΔEC/ΔEC embryos. dicerΔEC/ΔEC embryos display blood-filled structures contrary to WT embryos as revealed after hematoxylin/eosin staining (HE) (upper panel). Immunostaining with the lymphatic marker VEGFR-3 confirmed the lymphatic identity of the blood filled structures (lower panel). Cv: cardinal vein, ls: lymph sac. Scale bar: 50 μm. (n = 2 for each condition). B) Whole-mount view of a E14.5 dicerΔEC/ΔEC embryo after dissection (Left panel). Hemorrhages are indicated by Δarrows. Immunohistochemical staining by anti-LYVE-1 antibody on the same mutant embryo (Right panel). Scale Bar: 500 μm. Higher magnification of the dicerΔEC/ΔEC embryo after dissection and after LYVE-1 staining respectively (Lower panel). There is a complete overlap between hemorrhages and LYVE-1 staining indicating blood-filled lymphatics in the dicerΔEC/ΔEC embryo. (n = 2).