Figure 5

iMAEC-WT maintains shear-sensitive endothelial phenotype. iMAEC-WT were exposed to LS or OS or kept for static for 24 h. (A) iMAEC-WT aligned in the direction of the flow when exposed to LS but not OS or static control. Arrows indicate the imposed flow direction. (B) Total cell lysates were collected and protein level of KLF2 and eNOS was measured by Western blot. Data were shown as means ± standard error, n = 3. (C-F) Expression of shear-sensitive genes and microRNA under LS and OS for 24 h using early and late passages of iMAEC cells. iMAECs from early and late passages were subjected to either LS or OS for 24 h and expression of VCAM1, KLF2 and Interleukin-8 (C, D, F) was determined by qPCR and normalized to 18S. (E) Graph shows the expression of shear-sensitive miRNA, miR-712 as determined by qPCR and normalized to RNU6B. Cells under static condition served as control. * p < 0.05 compared to respective LS controls.