Characterization of iMAEC lines by immunostaining. iMAEC lines including iMAEC-WT, iMAEC-cav1, iMAEC-eNOS, and iMAEC-p47 were immunostained using the endothelial markers, PECAM-1, VE-Cadherin, and von Willebrand factor (vWF). Smooth muscle cell α-actin (α-SMA) was used as negative marker. HUVEC served as a positive control while 3T3 and RASMC were negative controls. Scale bar = 50 μm.