The effects of Notch inhibition with DAPT on Sox10 expression in A7r5 and A10 cells. A. Quiesced A10 cells were grown in media containing 10% FBS supplemented with or without 10 μM DAPT for 3 d before the expression of Sox10 was determined by immunocytochemistry. B. Relative gene expression of SMC markers [(SMA, MHC, CNN1 and smoothelin (SMO)] and neural stem cell MVSC markers [Sox10, Sox17 and S100β)] in A10 and A7r5 cells was determined by qRT-PCR. GAPDH was used to normalize gene expression. Data are mean ± SEM and are representative of three independent wells, *p < 0.01 when compared to 0.5% FCS.