SMC and stem cell marker expression in A10, A7r5 and MVSCs. A. Representative immunoblots for SMC differentiation markers (SM-MHC, SMA and CNN1) and neural stem cell markers (Sox10, Sox17, S100β in lysates from quiesced cells grown in normal DMEM media supplemented with FBS for 3 d. The levels of SM-MHC (Sm1, Sm2 and non-muscle MHC), SMA, CNN1, Sox10, Sox17 and S100β were determined using specific antisera against these antigens. Data are representative of blots with similar results. Equal loading was confirmed by Ponceau S staining of the membranes and by measuring the constitutive β-actin gene. B. Immunocytochemical staining of c-Kit and Flt-1 in MVSCs, rSMC, A7r5 and A10 cells. Quiesced cells were grown in normal DMEM media supplemented with FBS for 3 d before the cells were stained for c-kit and flt-1. C. Representative flow cytometry analysis of Flt-1 and c-Kit in A7r5, A10 and MVSCs using antibodies against Flt-1 and c-Kit. Open curves represent negative control samples; red filled curves represent samples stained with antibodies.