MVSC marker expression in A10 cells. A. Representative immunocytofluorescence of neural stem cell markers (a-c) Sox10, (d-f) SM-MHC (g-i) Sox17 and (j-l) Sca1 in quiesced cells grown in normal DMEM media supplemented with FBS for 3 d and visualised by (a-c) immunocytochemistry (d-i) confocal microscopy. Arrows show localisation of Sox10 and Sox17 primarily to the nucleus. The nuclei were stained with DAPI. Scale bar applies to all images, 250 nm except (a-c, 100 nm). Data are representative of three individual slides. B. Representative flow cytometry analysis of A10 cells with antibodies against SM-MHC, Sox10, Sox17, S100β and Sca1. Open curves represent negative control samples; red-filled curves represent samples stained with antibodies against SM-MHC, Sox10, Sox17, S100β and Sca1.