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Figure 4 | Vascular Cell

Figure 4

From: Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells?

Figure 4

MVSC marker expression in A10 cells. A. Representative immunocytofluorescence of neural stem cell markers (a-c) Sox10, (d-f) SM-MHC (g-i) Sox17 and (j-l) Sca1 in quiesced cells grown in normal DMEM media supplemented with FBS for 3 d and visualised by (a-c) immunocytochemistry (d-i) confocal microscopy. Arrows show localisation of Sox10 and Sox17 primarily to the nucleus. The nuclei were stained with DAPI. Scale bar applies to all images, 250 nm except (a-c, 100 nm). Data are representative of three individual slides. B. Representative flow cytometry analysis of A10 cells with antibodies against SM-MHC, Sox10, Sox17, S100β and Sca1. Open curves represent negative control samples; red-filled curves represent samples stained with antibodies against SM-MHC, Sox10, Sox17, S100β and Sca1.

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Vascular Cell

ISSN: 2045-824X

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