Figure 3

MVSC transition to vSMC following inductive stimulation. Representative immunocytofluorescence of SMC differentiation markers (a, b) SM-MHC, (c, d) SMA and (e, f) CNN1 in MVSCs cultured in differentiation media (DMEM supplemented with 10% FBS) for 7 d in the absence or presence of 2 ng/ml TGF-β1 and 10 ng/ml PDGF. The nuclei stained with DAPI. Scale bar, 100 nm.