Multipotent vascular stem cell (MVSC) marker expression. A. Representative immunocytofluorescence of SMC differentiation markers (a-c) SM-MHC and neural stem cell markers (d-f) Sox10, (g-i) S100β and (j-l) Sox17 in markers rat aortic MVSCs isolated by explant and cultured in MVSC maintenance media. The nuclei of were stained with DAPI. Scale bar, 100 nm. Data are representative of three experiments. B. Representative flow cytometry analysis of MVSCs cultured in DMEM supplemented with 10% FBS for 10 d with antibodies against SSEA-1, Sox10, Sox17 and S100β. Open curves represent negative control samples; red filled curves represent samples stained with antibodies against SM-MHC, Sox10, Sox17 an S100β.