Figure 2

Expression levels of Ang-2 and VEGF in different engineered CNE2 cell groups. (a): mRNA expression was determined by qRT-PCR. All the PCR amplification was performed in triplicate and repeated in three independent experiments. The relative quantities of selected mRNAs in cell samples were normalized to that of GAPDH. Higher Ang-2 mRNA expression was observed in C, D, and E (**P < 0.01, vs. B, no significant difference among A, B and F). Lower VEGF mRNA expression was observed in group E and F (## P < 0.01, # P < 0.05, vs. A, B, C, D). (b): Western blots of the above samples. (c): Densitometric quantitation of the band intensities shown in (b). The densitometric intensity of the target band in each lane relative to the intensity of the GAPDH band from the same sample was calculated, and plotted. Data were expressed as means ± SD of three independent experiments. The level of total Ang-2 protein in the Ang-2-transfected group C,D,E was significantly higher than that in control group B (*P < 0.05 vs. B; no significant difference among A, B and F), and the level of endogenous VEGF protein in the VEGF miRNA-transfected group E,F was significantly decreased (## P < 0.01, vs. A, B, C, D). The ratio of Ang-2/VEGF was significantly higher in E and F than in other groups.