Effects of VEGF silencing on CNE2 cells. CNE2 cells were treated with the transfection reagent only (mock), a scrambled miRNA (scrambled miRNA), or a VEGF-specific miRNA (VEGF miRNA). (a): Real-time RT-PCR demonstrated low VEGF mRNA expression in VEGF-interfered CNE2 cells. *P < 0.05, compared with the mock and scrambled miRNA groups. (b): Western blotting demonstrated lower VEGF protein expression in the VEGF-interfered CNE2 cells.