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Table 1 Quantitation of mEGFP positive neointimal cells 28 days following ligation

From: Previously differentiated medial vascular smooth muscle cells contribute to neointima formation following vascular injury

Myh11cre % mEGFP + Averaged from:
Mouse #   
1 76 ± 9 1827 cells (18 stacks, 10 sections)
2 86 ± 4 600 cells (5 stacks, 3 sections)
3 80 ± 11 773 cells (12 stacks, 8 sections)
4 90 ± 5 653 cells (6 stacks, 4 sections)
5 92 ± 4 227 cells (5 stacks, 3 sections)
6 42 ± 10 517 cells (5 stacks, 3 sections)
7 85 ± 7 463 cells (10 stacks, 8 sections)
Mean 79 ± 17 5060 cells (61 stacks, 39 sections)
Acta2 cre % mEGFP+ Averaged from:
Mouse#   
8 91 ± 4 731 cells (5 stacks, 3 sections)
9 72 ± 13 208 cells (8 stacks, 6 sections)
10 63 ± 16 189 cells (10 stacks, 6 sections)
11 74 ± 4 261 cells (6 stacks, 3 sections)
12 81 ± 7 561 cells (5 stacks, 4 sections)
13 94 ± 7 759 cells (8 stacks, 8 sections)
14 92 ± 3 190 cells (4 stacks, 4 sections)
Mean 81 ± 12 2899 cells (46 stacks, 34 sections)
  1. In order to count the number of mEGFP and mTomato positive cells within the neointima of each vessel (the cells between the internal elastic lamina and the endothelial lining of the lumen), a Z-stack series of 1 μm optical sections (XY-210 μm × 210 μm) were obtained from each vessel. mEGFP and mTomato positive cells were counted in each of the optical sections through the entire Z-stack of images, each DAPI positive nuclei was scored as being associated with either an mEGFP or mTomato positive cell. The number of total cells counted, together with the number of separate Z-stacks and sections counted are indicated. From some sections we were able to obtain 2 separate fields of Z-stack images, in others a single field encompassed the entire neointima.
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Vascular Cell

ISSN: 2045-824X

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