Figure 7

CD68 positive macrophages/monocytes cells can be seen within early developing neointima in some mice. Sections from the same two mice shown in Figure 6 were stained with antibodies to CD68 (white). Left panels show mTomato (red)/mEGFP (green) co-stained sections and in the right panels are the same sections visualized for mEGFP (green) and CD68(white). CD68-positive cells can be seen within the neointima of vessels from the mouse shown in the upper panels but not in the vessels of the mouse shown in the lower panels. Nuclei were visualized by staining with DAPI (blue). All images are shown at the same magnification with the scale bar shown in the bottom left panel representing 50 μm.