Skip to main content
Figure 3 | Vascular Cell

Figure 3

From: Previously differentiated medial vascular smooth muscle cells contribute to neointima formation following vascular injury

Figure 3

Tissue specificity of Acta2 cre ER(T2) activity. 5-week old Acta2 creER(T2)-/+ mTmG-/+ mice were treated with tamoxifen (1 mg,IP) once a day for 5 days. 2 weeks following the last tamoxifen injection the left carotid artery was ligated. 4 weeks later tissues were harvested and analyzed by confocal microscopy as described in ‘Methods’. A strong mEGFP signal can be seen only in smooth muscle cells of all the tissues examined. No cre activity was detected in other cell types including skeletal muscle cells, cardiac myocytes, endothelial cells, mucosal epithelial cells and hepatocytes. A heterogeneous staining was observed in the uterus suggesting that cre was not active in all the uterine smooth muscle cells during the period in which the mice were treated with tamoxifen. The patchwork mEGFP expression observed in the esophagus reflects the mixed skeletal/smooth muscle lineage of cells within the wall of this portion of the esophagus. SM-smooth muscle, LM-longitudinal muscle, CM-circular muscle, MP-myenteric plexus, MM-muscularis mucosa. Scale bars represent 40 μm in all panels.

Back to article page

Vascular Cell

ISSN: 2045-824X

Contact us