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Figure 11 | Vascular Cell

Figure 11

From: Previously differentiated medial vascular smooth muscle cells contribute to neointima formation following vascular injury

Figure 11

No mEGFP positive circulating cells can be detected following carotid ligation. Blood was harvested from Myh11 creER(T2)-/+ mTmG-/+ mice 7, 14 and 28 days following carotid ligation and subjected to fluorescence activated cell sorting. Cells harvested from a CAG-EGFP mouse were used as positive controls for EGFP expressing cells, those harvested from mTmG reporter mice with no cre transgene as positive controls for mTomato positive cells and those harvested from nontransgenic mice as negative controls. From each mouse a minimum of 10,000 cells were sorted. Upper panels show the distribution of mTomato (red) and mEGFP (green) positive as well as negative (black) cells. Numbers on each graph show the percentage of negative cells in each quadrant. Lower panels show the distribution of cells obtained from duplicate Myh11 creER(T2)-/+ mTmG-/+ mice. In each case the percentage of EGFP-positive cells are less than background negative control levels.

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Vascular Cell

ISSN: 2045-824X

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