Figure 2

CSF-1R^highcells exhibit increased phagocytosis. Phagocytosis of FITC-conjugated, IgG-coated latex beads was assessed in (A) CSF-1R^low and CSF-1R^high cells in hemangiosarcoma cell lines and (B) in the angiosarcoma cell line, AS5. Phagocytosis is represented as relative fluorescence intensity by each cell line. The macrophage cell line, RAW264.7, was used a positive control for the assay. The increased phagocytic activity in the CSF-1R^high populations from the hemangiosarcoma cell lines was significant (COSB, p* = 0.003 DD-1, p* = 0.034) compared to the activity detected in CSF-1R^low populations. Statistical significance was not detected (p = 0.08) between the CSF-1R^low and the CSF-1R^high cell populations enriched form the AS5 cell line.