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Figure 2 | Vascular Cell

Figure 2

From: VEGFR-1 blockade disrupts peri-implantation decidual angiogenesis and macrophage recruitment

Figure 2

VEGFR-1, CD31 and F4/80 expression in the non-pregnant uterus. IHC and double staining IF were performed on non-pregnant uterine cross-sections. (A) Schematic representation of a non-pregnant mouse uterus showing lumen (arrowheads), endometrial glands, stroma (s), and myometrium (myo). (B) ECs, detected by CD31 staining (brown), are observed throughout the stroma and myometrium. (C) Macrophages, detected by F4/80 staining (brown), are observed throughout the stroma and myometrium. (D) Double-staining for VEGFR-1+ (red) and CD31+ (green) demonstrates expression of VEGFR-1 in a subset of CD31+ ECs throughout the stroma. (E, F) VEGFR-1+ cells (red) and F4/80+ macrophages (green) are distributed throughout the stroma. VEGFR-1 and F4/80 co-expression is not observed. The inset (F) highlights contact of adjacent VEGFR-1+ cells and F4/80+ macrophages. (G, H) IF controls with secondary antibodies alone show no specific staining. L, lumen; g, endometrial gland. (B, C) Scale bar = 100 μm. (D-H) Scale bar = 50 μm.

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