Table 1 Primers used for RT-PCR and qPCR
From: Netrin-4 promotes mural cell adhesion and recruitment to endothelial cells
Name | Accession number | Forward primer | Reverse primer |
|---|---|---|---|
Neogenin | 62U612 | 5'-TCGCTGCGTAGTGGAAAGTG-3' | 5'-CTGTGTCAAGTGCCTCCTCA-3' |
DCC | X76132 | 5'-ACTTGGGGTGGTGAAGTCAG-3' | 5'-CCAAGACAGGGACCACATCT-3' |
Unc5A | AK131380 | 5'-CATCGAGTGCTTTGAGGTGA-3' | 5'-ACCTGCTGCCTTGAGACATT-3' |
Unc5B | AY126437 | 5'-ACTCATCTGCTGCCCTGACT-3' | 5'-ATTTTGTCGGTGGAGTCCTG-3' |
Unc5C | AF055634 | 5'-CACTGCACCTTCACTCTGGA-3' | 5'-GAGAGGGATGCTGAAAGCAC-3' |
Unc5D | AB055056 | 5'-GGTGCTCCTGAGTCCTGAAG-3' | 5'-GGGTCCAAAAGGCAGTAACA-3' |
Netrin 4 | AF278532 | 5'-CAGACATAGACTGGTGTCATGAAGTT-3' | 5'-ACATTGACCTCAACATGAGTACCTT-3' |
Beta actin | X00351 | 5'-AGAGCTACGAGCTGCCTGAC-3' | 5'-AGCACTGTGTTGGCGTACAG-3' |